Date: Wed, 5 Aug 2020 15:34:28 +0000 From: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Cytology Message-ID: <903be99abb344593a73079761d762...@phx-exch-013.chw.edu> Content-Type: text/plain; charset="us-ascii"
Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides. Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated. Hello Karen, Long time no see! I always used Charged slides, and prefixed Methanol for ~ 5 min @ RT (not 95% alcohol) before staining (SS & IHC). Hope this helps! *Madeleine Huey, B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94040 Phone: 650-940-7038 Fax: 650-988-8387 Email: madelein...@elcaminohospital.org On Wed, Aug 5, 2020 at 10:14 AM <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. process formalin-fixed tissues from animals infected with a > virus (Amy Lee) > 2. Update info. on old topic? > (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) > 3. Re: Update info. on old topic? (Paula Keene Pierce) > 4. MMR IHC validaton (Waitts, Celeste) > 5. Cytology (Heckford, Karen - SMMC-SF) > 6. Re: Update info. on old topic? (Morken, Timothy) > 7. Exciting New Histology Opportunities!! (Pam Barker) > 8. Quick question about Florida licensure. (Pam Barker) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 4 Aug 2020 11:13:42 -0700 > From: Amy Lee <amyleehisto...@gmail.com> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] process formalin-fixed tissues from animals > infected with a virus > Message-ID: > <CAGnbBwBxD9+uK7LyTdXVVBz0EGVcciTTR= > odxm+2y9gnbc+...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > Does anyone know what we should know about process formalin fixed animal > tissue that has been infected with virus? What > certificate/requirement/qualification we should have? We have never done > this before and need to make decision if we should do it or not. > > Thank you very much, > > Amy > > > ------------------------------ > > Message: 2 > Date: Wed, 5 Aug 2020 12:39:53 +0000 > From: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" <j...@cdc.gov> > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Update info. on old topic? > Message-ID: > < > sa9pr09mb5325cbde76121b31c6309f2380...@sa9pr09mb5325.namprd09.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) > Centers for Diseases Control and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > > > ------------------------------ > > Message: 3 > Date: Wed, 5 Aug 2020 13:00:37 +0000 (UTC) > From: Paula Keene Pierce <pa...@excaliburpathology.com> > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu>, "Sanders, Jeanine > (CDC/DDID/NCEZID/DHCPP)" <j...@cdc.gov> > Subject: Re: [Histonet] Update info. on old topic? > Message-ID: <1398404991.167739.1596632437...@mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > I am interested in this too, with my main question being the quality of > digital images from film coverslipped slides. > Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 > N Blue Lake DriveNorman, OK 73069PH 405-759-3953 > http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > > On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine > (CDC/DDID/NCEZID/DHCPP) via Histonet <histonet@lists.utsouthwestern.edu> > wrote: > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) > Centers for Diseases Control and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Wed, 5 Aug 2020 15:22:48 +0000 > From: "Waitts, Celeste" <cwai...@centrastate.com> > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] MMR IHC validaton > Message-ID: > <8c30fb230afd42f7a54c520887028...@exchnode1.local.centrastate.com> > Content-Type: text/plain; charset="us-ascii" > > HI, > WHAT is everyone's opinion on the best way to validate the Roche MMR IHC > panel. Cost, time, ease? > Any help? > Thank you > Celeste Waitts > Histology Supervisor > Centrastate Medical Center > > > > > ------------------------------ > > Message: 5 > Date: Wed, 5 Aug 2020 15:34:28 +0000 > From: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Cytology > Message-ID: <903be99abb344593a73079761d762...@phx-exch-013.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I rarely do Special stains or IHC's on Cytology cytospins or thin prep > type slides. Should these slides be charged, air dried or fixed in 95% > alcohol before doing Special stains or IHC's on them? > > Is there a good book or some sort of publication on cytology procedures > regarding the above mentioned. I have tried and look it up and it seems > all over the place on what to do. > > Any help would be greatly appreciated, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > > > ------------------------------ > > Message: 6 > Date: Wed, 5 Aug 2020 15:38:14 +0000 > From: "Morken, Timothy" <timothy.mor...@ucsf.edu> > To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" <j...@cdc.gov> > Cc: Histonet <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Update info. on old topic? > Message-ID: > < > byapr05mb57039195dfe1e6c67b67b1f7e7...@byapr05mb5703.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Jeanine! > > We are using film coverslipping exclusively now and scanning all slides. > We moved to film because it dries almost instantly and can be scanned right > away. It has worked very well. The only issues, and not specific to film > coverslipping, are that some slides with low contrast sometimes are out of > focus. That is primarily IHC slides with low contrast counter stain and low > contrast specific staining. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet < > histonet@lists.utsouthwestern.edu> > Sent: Wednesday, August 05, 2020 5:40 AM > To: 'histonet@lists.utsouthwestern.edu' <histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Update info. on old topic? > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control > and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=rzbMGLf4Y17ghrmD701AEURtk3lAZ5QHWvK_mUBrhTs&s=NdAh9f9sPJR8Cc0amnB3m1WH4qmQ7DH1q5uQoy7e2k4&e= > > > > ------------------------------ > > Message: 7 > Date: Wed, 5 Aug 2020 12:14:21 -0400 > From: "Pam Barker" <rel...@earthlink.net> > To: "Histopeeps Histonet" <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Exciting New Histology Opportunities!! > Message-ID: <000001d66b43$7b758ad0$7260a070$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters, > I hope you are having a great week. > I wanted to send a quick note to tell you about the positions that I am > working on and am most excited about.? > Why am I excited about these positions? > Because each of these clients was asked if I had someone for you today > would > you be ready to interview and hire? > Every One of these clients said YES!!! > Here are the Super Sizzlin Hot Opportunities! > > Leadership Opportunities: > North Carolina ? Greensboro > North Carolina - Concord > > Histology Tech, IHC and Mohs Tech Opportunities > N. Carolina Charlotte > N. Carolina Wilmington ? Mohs > N. Carolina Wilmington > Florida Sarasota area > California - Modesto ? IHC > California Modesto > > All of these positions are full time and permanent!! > My clients offer excellent compensation, benefits and in most cases either > relocation or a sign-on bonus. > > > Histopeeps, If you think you or someone you know might be interested in any > of these opportunities or would like to talk about a job search in another > area, please contact me. > If I place someone you refer You will earn a referral fee. > > If you are interested in any of these opportunities > CALL /TEXT MY CELL ASAP!!! At 407-353-5070. > > > I can also be reached toll free at the office at 866-607-3542 or at > rel...@earthlink.net to set up a time to talk at your convenience! > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > *15 Years!* > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > ?Pam M. Barker? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: rel...@earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Aug 2020 12:19:28 -0400 > From: "Pam Barker" <rel...@earthlink.net> > To: "Histopeeps Histonet" <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Quick question about Florida licensure. > Message-ID: <001701d66b44$323b8d00$96b2a700$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histopeeps! > I have a quick question about Florida licensure. Can someone who holds a > MLT(ASCP) get licensed and work as a histotech in Florida? > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > *15 Years!* > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > Pam M. Barker > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: rel...@earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 201, Issue 3 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet