Hi Tim, From a user perspective: The slides you’re using may be a bad lot causing hydrophobic staining or you’re not cleaning your covertiles correctly. The wells on the assembly might also not be pulling adequately. Have you cleaned the assembly recently, particularly the wells? You could use a clean kit offer by Novocastra or make your own. Performing a decontamination on your instrument would be helpful too to include your bulk reagent compartments and reagent syringe. Have you also been performing the suggested manufacturer’s guidance on maintaining your Bond-Max IHC/ISH analyzer? Or... Simply the instrument needs to be recalibrated by a Leica engineer. Do not PM with a third party biomed group as the Leica engineer will need to recalibrate the instrument with a PM kit to check and if necessary replace the physical components, electronics, and fluidic system of your analyzer along with updating the coordinate system to match software build according to their latest recommendation from their home office.
Apart from typical lab reagents or processes only performed by an engineer, do you have electrical or magnetic surges in your lab? The backup battery that comes with your Bond is typically good for two years, and would need to be replaced every few years, especially as the Bond-Max requires a step down from the 120V outlet to 110V. And this consideration can also deeply impact your overall staining. The application specialist should have more politely said that they didn’t know but will try to get a response back to you. I don’t comprehend how the tray would be the issue unless it was severely cracked and would prevent the slide assembly from functioning properly. Very respectfully, Eddie Martin, HT, HTL, QIHC Hematology Team Leader The National Institutes of Health 10 Center Drive Bethesda, MD 20892 Sent from my iPhone > On Aug 13, 2020, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: End of shift (Charles Riley) > 2. Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy) > 3. Re: Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy) > 4. Re: Blank spots on IHC tissues - bubbles on Bond > (Paula Keene Pierce) > 5. Protocol or SOP of how to make Tissue microarray > (Chakib Boussahmain) > <mime-attachment> > <mime-attachment> > <mime-attachment> > <mime-attachment> > <mime-attachment> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
