Hello Merissa, You can use a diamond band saw, as they are produced by EXAKT. This special technique will cut soft tissue quite smooth without destroying the tissues. So you can cut slices with a few centimeter thickness which can be fixed in formaline after sectioning.
What kind of embedding and sectioning / staining you are planing? You can embed the tissues some special resins which are suitable for larger specimens. If you need more information about the resin techniques and pathology saws, please contact me or Jerrod Roberts from EXAKT USA. Kind regards Michael > Am 21.08.2020 um 17:37 schrieb M.O. via Histonet > <histonet@lists.utsouthwestern.edu>: > > Happy Friday, Histonetters! > > I am doing some planning for a new project and wanted to get opinions on > fixation of large pieces of tissue. We will have human shoulders, where we > want to preserve the rotator cuff/joint. Cutting the tissue with a saw will > damage the soft tissue, so we were thinking that post-fixation would be > best for cutting slabs. > > Does anyone have experience with fixing such large pieces of tissue? We > typically use zinc buffered formalin for fixation. Would a vacuum work or a > vacuum sealer? > > Thank you for any input you might have!!!! > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de <mailto:michael.g...@morphisto.de> Internet: http://www.morphisto.de/ <http://www.morphisto.de/> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
