Hi martha, Prognostic markers must be re-validated (Eg.s Breast markers and CD117) as you described. Every Ab in your menu should be tested (as you would for a new a new lot) and do not forget to validate your H&E (with various tissue types) and SS as well. For the H&Es, if possible do side-by-side comparisons between old and new processors (the downside of what is otherwise an exciting time!). Greg
-- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
