We use poly-L-lysine. Works for us. P4707 Sigma-Aldrich Poly-L-lysine solution 0.01%, sterile-filtered, BioReagent, suitable for cell cultur
Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 charles.ba...@baystatehealth.org -----Original Message----- From: Charles Riley <cri...@dpspa.com> Sent: Friday, November 13, 2020 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toenail staining issues >From time to time, we have issues with our sections of toenail falling off the >slide during staining. Our H&E sections usually stain without issue but when we do our special stains (PASF and GMS) the tissue falls off routinely. We use charged slides and if a section falls off, we coat the recut slide with albumin to help get extra adherence. However sometimes even this does not help the tissue stick through the whole process (especially on GMS). Does anyone have any additional suggestion we could try to get the sections to adhere better throughout the process? ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
