Interesting thread. Thanks for kicking it off.

Does anybody have a reference that cites diagnostic errors caused by floaters / 
contamination from other specimens?

John

On Mon, Dec 7, 2020 at 9:15 AM, Joe W. Walker, Jr. via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:

> Don’t shoot the messenger. :)
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology and Interim Phlebotomy Manager
> Rutland Regional Medical Center
> 160 Allen Street, Rutland, VT 05701
> P 802.747.1790 F 802.747.6525
> joewal...@rrmc.org, www.rrmc.org
>
> -----Original Message-----
> From: Terri Braud via Histonet <histonet@lists.utsouthwestern.edu>
> Sent: Monday, December 7, 2020 8:42 AM
> To: 'histonet@lists.utsouthwestern.edu' <histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Floaters
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don’t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don’t 
> recognize the sender.
>
> 60% of floaters from the water bath? I find that really hard to believe.
> The Gephardt and Zarbo CAP study from 1996 showed reported the results of a 
> Q-Probes study of 275 laboratories and documented a frequency of 
> contamination of between 0.6% and 2.9%, depending on the study method. Their 
> study demonstrated the rate of extraneous tissue contamination was higher for 
> blocks than for slides and higher in a retrospective review than in a 
> prospective study. So in other words, when people knew they were being 
> studied, they were more careful and the contamination rate went down, but in 
> retrospect, the majority of floaters occurred in the blocks, not the water 
> bath.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> HNL Labs, Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> Today's Topics:
>
> 1. "Floaters" in surgical or cytology specimens
> (Martha Ward-Pathology)
> 2. Re: "Floaters" in surgical or cytology specimens
> (Joe W. Walker, Jr.)
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 4 Dec 2020 13:55:20 +0000
> From: Martha Ward-Pathology <mw...@wakehealth.edu>
> To: "histonet@lists.utsouthwestern.edu"
> <histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] "Floaters" in surgical or cytology specimens
> Message-ID:
> <b2cecb1b6665a4479056478f6de3c4ab01ad767...@exchdb6.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I am posting this question for a colleague in our Cytology department. How 
> often do you see floaters on surgical or cytology specimens? Obviously we 
> would never want to see any type of carryover but is there a standard rate 
> published somewhere that he can reference?
>
> Thanks in advance for your help.
>
> Martha Ward, MT ASCP, QIHC
> Manager, Molecular Diagnostics Lab
> Wake Forest Baptist Medical Center
>
> ------------------------------
>
> Message: 2
> Date: Fri, 4 Dec 2020 17:41:11 +0000
> From: "Joe W. Walker, Jr." <jwwal...@rrmc.org>
> To: Martha Ward-Pathology <mw...@wakehealth.edu>
> Cc: "histonet@lists.utsouthwestern.edu"
> <histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens
> Message-ID:
> <bl0pr11mb3155934b94c32b5358af2485bb...@bl0pr11mb3155.namprd11.prod.outlook.com>
>
> Content-Type: text/plain; charset="utf-8"
>
> https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$
>
> "Floaters represent a potential source of diagnostic error and occur in 0.01% 
> to 1.2% of slides. Pick up of floaters from the water bath appears most 
> common (?60%). Floaters in only 1 level and mismatch with the specimen tissue 
> type are clues to the extraneous nature of the floater."
>
> Joe W. Walker, Jr. MS, SCT(ASCP)
> Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical 
> Center
> 160 Allen Street, Rutland, VT 05701
> P 802.747.1790 F 802.747.6525
> joewal...@rrmc.org, http://www.rrmc.org
>
> -----Original Message-----
> From: Martha Ward-Pathology via Histonet <histonet@lists.utsouthwestern.edu>
> Sent: Friday, December 4, 2020 8:55 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] "Floaters" in surgical or cytology specimens
>
> [External Email] This email originated from outside of the organization. 
> Think before you click: Don?t click on links, open attachments or respond to 
> requests for sensitive information if the email looks suspicious or you don?t 
> recognize the sender.
>
> I am posting this question for a colleague in our Cytology department. How 
> often do you see floaters on surgical or cytology specimens? Obviously we 
> would never want to see any type of carryover but is there a standard rate 
> published somewhere that he can reference?
>
> Thanks in advance for your help.
>
> Martha Ward, MT ASCP, QIHC
> Manager, Molecular Diagnostics Lab
> Wake Forest Baptist Medical Center
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