Kelly, You are not going to stay within the temp ranges if putting pellets directly into the embedding center tank. A couple solutions: 1) write into your procedure that the temperature will go down when pellets are added and that once melted should be in whatever range it calls for (and don't turn it up, because that means you have to keep adjusting it and someone may forget and as you say, exceed the temperature - you just need to put in far enough ahead to let it melt. Or, 2) Get an bulk paraffin dispenser and melt the paraffin in that, then draw off liquid paraffin for your embedding center. This is pretty common for tissue processor and embedding centers to avoid having to use them to melt the paraffin. Gets you back to work faster as well.
Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: E. Wayne Johnson via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, December 08, 2020 1:26 PM To: Pairan, Kelly <kelly.pai...@nationwidechildrens.org>; 'histonet@lists.utsouthwestern.edu' <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks You could warm the paraffin in an oven overnight to melt it so that the heat of fusion is not extracted from the embedding center paraffin tanks when it is added as melted paraffin at 58-65 degrees. Pairan, Kelly via Histonet wrote: > Good Afternoon, > Recently we starting taking the daily maximum and minimum temperature ranges > for our embedding center paraffin tanks. The melting point of our wax is > 56oC and our current temperature range is 58 to 65oC. The problem we are > having is that when we add wax, the temp dips below the range until it melts. > If we turn it up, it exceeds the max range. Any suggestions? > > Thanks, > Kelly > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7 > cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=4_M_9rKMFL-Sh7pBndIIL-bv0 > oW9Qdhfz4CMKoyUJz0&s=sXXQcK8dPXYAYVBhgQQkD_qJtcBV3mOUyb7ehqCprQg&e= > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=4_M_9rKMFL-Sh7pBndIIL-bv0oW9Qdhfz4CMKoyUJz0&s=sXXQcK8dPXYAYVBhgQQkD_qJtcBV3mOUyb7ehqCprQg&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
