I use charged slides all the time. The best way to clear the water is to cut the slides and air dry them overnight. The next day I follow the normal protocol, heat in 60 degree over and then on to the rest of the protocol.
They don't really need the 56 degree oven all night long. Respectfully, Colleen Forster On Mon, Jan 25, 2021 at 11:26 AM Tyrone Genade via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > > On Saturday, January 23, 2021 1:00 PM, < > histonet-requ...@lists.utsouthwestern.edu> wrote: > > > In my lab, water under the sections is unique to charged slides. > > And you > > are correct, if there is water under the section when the slides > > are heated > > for antigen retrieval, the boiling (or at least very hot) water > > will damage > > or entirely destroy the section. > > I had this very problem and the simple solution was to bake the slides > overnight at 56oC (melting temp of the wax I was working with). I would > still heat on a hot plate before dewaxing. Baking at 45oC also seemed to > work. Whether this would work for you would depend on the sensitivity of > your epitopes to prolonged heat. > > Good luck... every now and then a section would still detach during > antigen retrieval. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet