I have to agree with Bob. Bouin's should not be used as an ink fixative, especially in a spray bottle. We use 5% Vinegar, or glacial acetic acid, and it works just fine. Just my 2 cents - T
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Sunday, February 07, 2021 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 207, Issue 5 CAUTION: This email originated from outside Holy Redeemer. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Bouin's fixative (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Sat, 6 Feb 2021 13:22:22 -0500 From: Bob Richmond <rsrichm...@gmail.com> To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Bouin's fixative Message-ID: <caoksrh6wwmz1utog1wbh1o9jtjjqvnswfnya9fqkyapotfe...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" > > Bouin's fixative has no business being used in a spray bottle. It's a very > toxic material that shouldn't be aerosolized. > As Dr. Steve McClain suggests, dilute acetic acid - 3 to 5%, white vinegar, is quite sufficient for preparing tissue for inking, and isn't toxic and messy like Bouin's fixative, or flammable like acetone. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 207, Issue 5 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
