Good Morning Heather, I have some questions about how you cut frozen brains.
What temperature are you cutting at? How thick are your sections? How are your samples frozen? Flash freezing, slow freezing, iso-pentane in LN2? Your answers may provide clues to help you get better cryosections. Paula Sent from my iPhone > On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hello Histonet, > > I'm looking into working up a tape transfer method of collecting > cryosections of brain while preserving infarct to be used in IHC. I > find that when I try and section heavily damaged regions of the brain > the tissue tears and and I lose it. Has anyone got any recommendations > about the the Section-lab transfer tape (Kawamoto method), using the > circuit plating tape recommended here > (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the > cryojane system from Leica? > > Thank you, > > Heather > > Heather Deziel, MSc. > > Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A > 4P3 > > (a subsidiary of Neurodyn Life Sciences Inc.) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
