Dear Alida, That is a good point. I probably have only done HOECHST on Pwax sections after they have been heat-Antigen retrieved ( HIER) However, H ( or DAPI) works on fixed cell monolayers: they don't get HIER treatment) Nor do frozen sections ( well, some do) and the HOECHST/DAPI works fine on both However, perhaps you can just take a Pwax section to water and apply HOECHST for 10 mins, wash off, mount in anti fade aq. mountant and have a look down a Fluorescence scope? If you can wait, I will check this next Monday ( put H on a non HIER'd Pwax section and get back to you by Monday later)
Unless, whoever that is doing the fluorescence DAPI/HOECHST nuclear staining is actually doing it on Pwax sections that have been HIER-ed? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
