Message: 1 Date: Wed, 14 Jul 2021 22:03:24 +0000 (UTC) From: Cheryl <tkngfl...@yahoo.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] PathCentre (ThermoShandon) protocols - help? Message-ID: <7393119.276682.1626300204...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8Help?? Starting a refurbished PathCentre and was hoping someone could share a biopsy protocol for xylene processing?? Routine human biopsies with specials and some IHC and molecular down the road. Open to all -? Much appreciated!! Cheryl Kerry, HT(ASCP)
Hi Cherryl it's a good machine (we have 3 of them) Here under some "official" protocole If unreadable, i'll send you pdf - just ask for it Richard Wild ----------------- SHANDON PATHCENTRE PROCESSING SCHEDULE RAPID BIOPSY PROGRAMME TOTAL DURATION 1hr 14mins STEP REAGENT CONC % TEMP °C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Formalin 10 45 V 00:01 15 S 2 Formalin 70 45 V 00:01 15 S 3 Alcohol 95 45 V 00:01 15 S 4 Alcohol 100 45 V 00:01 15 S 5 Alcohol 100 45 V 00:01 15 S 6 Alcohol 100 45 V 00:01 15 S 7 Alcohol 100 45 V 00:01 15 S 8 Xylene 45 V 00:01 15 S 9 Xylene 45 V 00:01 15 S 10 Xylene 45 V 00:01 15 S 11 Wax 60 V 00:01 60 S 12 Wax 60 V 00:01 60 S 13 Wax 60 V 00:01 60 S 14 Wax 60 V 00:01 60 S SHANDON PATHCENTRE PROCESSING SCHEDULE RAPID PROCESSING TOTAL DURATION 0hr 56mins STEP REAGENT CONC % TEMP °C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Formalin 10 45 V 00:02 15 S 2 Formalin 70 45 V 00:02 15 S 3 Alcohol 95 45 V 00:02 15 S 4 Alcohol 00:00 S 5 Alcohol 00:00 S 6 Alcohol 100 45 V 00:02 15 S 7 Alcohol 100 45 V 00:02 15 S 8 Xylene 00:00 S 9 Xylene 45 V 00:02 15 S 10 Xylene 45 V 00:02 15 S 11 Wax 60 00:00 S 12 Wax 60 00:00 S 13 Wax 60 V 00:02 60 S 14 Wax 60 V 00:02 60 S SHANDON PATHCENTRE PROCESSING SCHEDULE LARGE SURGICALS TOTAL DURATION 22hrs 34mins STEP REAGENT CONC % TEMP °C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Buffered Formalin 10 A N 02:00 15 S 2 Alcohol Formalin 10 A N 02:00 15 S 3 90% IMS * 60 A V 01:30 15 S 4 IMS 80 A V 01:30 15 S 5 IMS A V 01:30 15 S 6 IMS A V 01:30 15 S 7 Xylene/IMS A V 01:30 15 S 8 Xylene A V 01:30 30 S 9 Xylene A V 01:30 60 S 10 Xylene A V 01:30 60 S 11 Wax 60 V 01:00 120 S 12 Wax 60 V 01:00 120 S 13 Wax 60 V 01:00 120 S 14 Wax 60 V 01:30 120 S _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
