Of course you are right! This is yet another example of an error in a procedure informally handed on from person to person! Always work from a book. Even a very old one will be OK for paraffin embedding. John Kiernan. https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Sent: November 12, 2021 11:03 AM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Paraffin embedding following storage in 70% alcohol
I am working with a grad student on a project dealing with equine articular cartilage. The protocol she sent me for embedding the tissue samples goes directly from 70% alcohol to the embedding step in paraffin. Correct me if I am wrong but shouldn't the tissue be dehydrated fully and cleared before embedding the samples? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
