Jessica,
What has Leica said about the problem? I have always found them to be responsive and able to provide suggestions. This would be especially true with a new instrument, still under warranty. We have never had any inconsistencies with our Autocut, which was purchased last spring. Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Piche, Jessica via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, January 14, 2022 8:43 AM To: Histonet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] microtome blades and wax *** This is an External Email *** Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really having to work much harder to get a great section. We were wondering what everyone else uses. I was also wondering if the wax could be an issue. We use EM-400 for embedding. I'd appreciate anyone's thoughts. Thank you and have a nice weekend! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!uiyCL_cBeR_FRDl6tMCr34gjeUYcHEGHSOx1bIEuIcwqytIu5aQZnnf0pnjQJj2bw_sa$ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet