Like Amos, I have extensive experience in working with biotin/streptavidin (BSA) detection systems, and use them primarily because, in my analyses, such reagents cost about half as much as polymers kits in the same volume containers. In addition, knowing that endogenous biotin can be problematic in a small number of different tissues, our (clinical) lab simply avoids using BSA in those situations. As required by CAP standards, our IHC policy acknowledges that we’ve conducted the necessary testing to determine if/when endogenous biotin makes interpretation challenging, and refrain from doing so when applicable.
Finally, although I don’t agree with Carl’s belief that one MUST use the DAB chromogen+substrate components offered by the same vendor as the detection components, we DO purchase such a reagents from the same manufacturer simply because they’re more inclined to assist us in troubleshooting if we run into a problem with EITHER reagent. Sally On Sun, Feb 6, 2022 at 2:40 PM Hobbs, Carl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > As I stated: > Polymer systems only work GREAT when you also use the DAB in the kit > You pay dearly for it.... > Check out my comparison in Histonet Images > Carl > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Sally Price _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet