Hi, With regard to adhesion, we find TOMO slides are the best among many that we have tried.
Interestingly, a creative histotech I once worked with used a diluted Gorilla Glue smear on the slides just prior to picking up a cartilage section. It was tricky to get the section on while the glue was in a workable condition, but the adhesion was phenomenally improved. IHC worked very well on these slides. Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 www.arrayscience.com On Tue, Mar 1, 2022 at 11:30 AM Hobbs, Carl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Yes...a big problem for me, too....if you are using HIER?? > Cartilage and bone are major tissue retention problems > I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC > I still get a % fall off but, using manual HIER at 90C I manage to get > enough sections to get good results > However, I imagine that your autoIHC stainer prob uses 90C HIER? > I find Trajan 3 series slides more reliable than Superfrost Plus slides. > Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we > can't turn ours off/down) > Then I place slides in a 60C oven for 2hrs. > Then dewax etc.... > > Be interesting to read of other suggestions > > Best wishes > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet