Good info I have always made 1/10 stocks of my abs ( in TBS/BSA/azide) and frozen aliquoted neat stocks. For eg: I still use a 10 yr old "Dako" anti GFAP at 1/5000 ( stock 4C 1/10) Sure, I am in research Sure, I always add a positive control Today I used a 5 yr old 1/10 4C of anti PCNA ( Abcam ab29)...worked as well as when I 1st made the 1/10 stock Sure....one has to keep checking by adding controls ( a known positive like gut so that if crypt nuclei are exclusively +ve...OK) Mind you.....PCNA is NOT a great prolif marker : far better to use anti Ki67 ( half-life difference) However, I wanted to see if Ki67/PCNA was good for worm Pwax sections ( former -ve, latter TOO positive) Caveat: stored positive control sections can "go off" Some call it "oxidation" of epitopes It happens to some epitopes/ags Best wishes, Histonet
Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
