> > > I used a cryostat, a large, very primitive cryostate (long fleece gloves) > at Washington University in St. Louis in 1962, in a laboratory doing the > quantitative histochemistry techniques developed by Dr. Oliver Lowry before > then. We cut very thick sections, around 50 or 100 µm as I remember.
When I started my pathology residency at Johns Hopkins in 1964, we had the early International cryostats, refrigerated rectangular boxes containing a microtome. The old "wet knife" technique John Kiernan describes was completely out of use at Hopkins, though it remained in widespread use by surgical pathologists through the 1970s. I always refused to learn the technique, because the pathologists I saw using it shot from the hip, often calling cancer when it wasn't there. But I remember the old pathologists cutting the sections, floating them off into an ice cream dish, picking them up with a glass rod, staining them with Parker's blue-black ink, slapping them onto a slide and coverslipping in water. Bob Richmond Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet