I am working on trying to get IHC stains optimized on bone samples of rat tibias. The issue I am running into is that the periosteum is separating from the bone marrow and wrinkling up over top of the rest of the sample.
If anyone has any techniques to prevent this wrinkling/detachment of tissue from the main section during automated ihc staining it would be greatly appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet