We have not encountered a covertile artifact with our Bond III, which we've had for over 10 years. It may be that the covertiles need to be replaced or cleaned more, but we have not experienced this. We also place a control tissue at the top of the slide, and often have large patient sections on the lower portion. Rene had some good suggestions. I would also call Leica to see if they can offer suggestions over the phone. Their technical support team has been very helpful over the years. Best of luck.
------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Regan Fulton via Histonet <histonet@lists.utsouthwestern.edu> Sent: Thursday, September 29, 2022 3:42 PM To: RENE MORALES <rbasil...@sbcglobal.net> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC background *** This is an External Email *** Hi, The artifact you are seeing is a well-known artifact of the Leica covertile design. It does limit the available footprint for placing samples. Hope this helps. Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 https://urldefense.com/v3/__http://www.arrayscience.com__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxiUj3lZA$ On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Paula, > Regardless of the position of the tissue: control/ patient ‘s tissue, most > likely is the distribution of the reagents during staining, isn’t > adequate. Syringe, staining reagents expiration, calibration, etc., Have > you contacted service from Leica? Hope the solution is found, > Thanks, > > > Sent from my iPhone > > > On Sep 29, 2022, at 12:00, Paula via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > Hello everyone, > > > > > > > > We have the Bond III and we ran an S100. We placed 2 serial sections > > vertically on the slide with the control tissue at the top for staining. > The > > patient's section at the top underneath the control tissue had background > > staining, while the section on the bottom of that had no background > > staining. Can someone determine why this would happen? > > > > > > > > Thank you in advance. > > > > Paula > > > > Bio-Path Medical Group > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$ > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
