Hi Charles, protocols are going to depend on the species of your primary Abs you're looking to combine. If it's mouse and rabbit you can stain simultaneously. I use Biocare's double stain 1 or double stain 2 polymer depending on which marker you want on what colour.
If your Abs are the same species then you may need to stain sequentially with a denaturation step in between. Feel free to shoot me an email with the Abs you're using and I can help with some suggestions. Katy Milne MCIC-Histology, Deeley Research Centre BC Cancer Victoria -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: November-18-22 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 228, Issue 10 This email came from an EXTERNAL SENDER. If you think this message is suspicious, please do not open any attached files or links, and forward it as an attachment to spamm...@bccrc.ca Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Testing Skills (Terri Braud) 2. Manual double staining IHC (Charles Riley) 3. Histotechnician and Medical Technologist positions - Fred Hutchinson - Seattle (Donato, Elizabeth) 4. Re: Manual double staining IHC (Frazer Bell) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Nov 2022 18:16:51 +0000 From: "Terri Braud" <tbr...@holyredeemer.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Testing Skills Message-ID: <ef1beb923cd442c6b0435151aabfe...@holyredeemer.com> Content-Type: text/plain; charset="us-ascii" Aside from the terrible legal liability that you would open your institution to by asking them to operate "dangerous" equipment when they are not in your employ, sitting at a strange microtome/waterbath set up with different knives, paraffins, processing, just would not be an accurate test. I really liked Thomas Podawiltz's idea. Usually I just ask leading questions and then evaluate during the probation period. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ------------------------------ Message: 2 Date: Thu, 17 Nov 2022 14:25:50 -0500 From: Charles Riley <cri...@udel.edu> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual double staining IHC Message-ID: <CAHMJNChEW21NJMPoZuEFOP69n0fK0su=truk-k12f6rzvp3...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Hello all, I have never done double staining manually and wanted to know if anyone has protocols or suggestions for doing this? ------------------------------ Message: 3 Date: Thu, 17 Nov 2022 20:03:27 +0000 From: "Donato, Elizabeth" <edon...@fredhutch.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Histotechnician and Medical Technologist positions - Fred Hutchinson - Seattle Message-ID: <mw5pr11mb580916c4707ac1a800802fbaa3...@mw5pr11mb5809.namprd11.prod.outlook.com> Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I hope you will consider sharing these job postings with your colleagues. Fred Hutchinson Cancer Center is a wonderful organization to work for and the Clinical Trials Pathology lab in particular is involved in impressive, cutting-edge research. Thank you, Liz Research Histotechnician I/II https://careers-fhcrc.icims.com/jobs/23684/research-histotechnician-i-ii/job Clinical Research Technologist II https://careers-fhcrc.icims.com/jobs/23780/clinical-research-technologist-ii/job www.fredhutch.org<http://www.fredhutch.org/> Liz Donato Manager | Specialized Pathology Laboratory Clinical Research Division Fred Hutchinson Cancer Center ????????????????????????????????????????????????????????????????????????????????????????????? 206.667.4501 p | 425.443.6388 c | 206.667.5255 f | edon...@fredhutch.org<mailto:edon...@fredhutch.org> ------------------------------ Message: 4 Date: Fri, 18 Nov 2022 07:47:21 +0000 From: Frazer Bell <frazer.b...@glasgow.ac.uk> To: Charles Riley <cri...@udel.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Manual double staining IHC Message-ID: <lnxp265mb0458d7517dd61373ac8a22bbb1...@lnxp265mb0458.gbrp265.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hi, We have successfully done double staining using both DAB and Magenta on our Dako autostainer, you could do it manually. If you would like further information please let me know. Frazer Bell Histopathology Technician Histology Research Service/ Veterinary Diagnostics Services 307 Jarrett Building School of Biodiversity, One Health & Veterinary Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow G61 1QH Tel: +44 (0) 141 330 6933 email: vet-histol...@glasgow.ac.uk Website: http://www.gla.ac.uk/histology The University of Glasgow, charity number SC004401 -----Original Message----- From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Sent: 17 November 2022 19:26 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual double staining IHC Hello all, I have never done double staining manually and wanted to know if anyone has protocols or suggestions for doing this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 228, Issue 10 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
