Nancy, I would say that it can be done in a very particular situation: i.e.: no more tissue available, and type of IHC stain. If it is a dual stain, pathologist can not obtain the good results. In the lab I worked before, it was done, but this technique was not part of the protocol. Hope it helps, René
Sent from my iPhone > On Feb 8, 2023, at 10:35, Bacon, Charles via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hi Nancy, > > We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin > and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway > so we find this protocol effective. Then we remove the deparaffinization step > from our IHC protocol and run the slide. > > 1% Acid Alcohol > 70% Alcohol......99ml > HCL...................1ml > > Chuck Bacon, HTL(ASCP)CM > Supervisor Histology > Baystate Medical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > charles.ba...@baystatehealth.org > > -----Original Message----- > From: Cooper, Brian <bcoo...@chla.usc.edu> > Sent: Wednesday, February 8, 2023 12:46 PM > To: Nancy Schmitt <nancy.schm...@mercyhealth.com>; Histonet > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] destain for IHC > > We used to do this all the time in my old reference lab. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357<tel:+13233613357> > > bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu> > > ________________________________ > From: Nancy Schmitt via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Wednesday, February 8, 2023 9:29:13 AM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside > CHLA. Do not open attachments, click on links, or respond unless you expected > this message and recognize the email address: > histonet-boun...@lists.utsouthwestern.edu. > > Hello- > > Has anyone had success destaining an H&E and then running IHC on the same > slide? > > Thanks! > > Nancy > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and > is intended for the sole use of the intended recipient(s). It may contain > information that is privileged and confidential. Any unauthorized review, > use, disclosure, or distribution is prohibited. If you are not the intended > recipient, please delete this message, and reply to the sender regarding the > error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!f6gMMu3epLW9iWatLaXyHemacm0hDOoYOXCaMU5Ijo60vj-bxgHDrpUHZFZGfrf594v9R-jNYLfSmKM-MmAw_V6es7aYOBgKp9eltUXF9cY0L4I$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential or > legally privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > > ---------------------------------------------------------------------- > Please view our annual report at http://www.bhannualreport.org > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the designated > recipients named above. If you are not the intended recipient, you are hereby > notified that you have received this communication in error and that any > review, disclosure, dissemination, distribution or copying of it or its > contents is prohibited. If you have received this communication in error, > please reply to the sender immediately or by telephone at 413-794-0000 and > destroy all copies of this communication and any attachments. For further > information regarding Baystate Health's privacy policy, please visit our > Internet site at https://www.baystatehealth.org. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
