Hi Charles I have done this stain many times as an abbreviated version of Movats Pentachrome. You are correct in thinking the van gieson will affect the AB stain due to the acid.
As in the Movat stain do AB first then place slides in alcoholic ammonia for 1 hour to convert AB to insoluble Monastral blue then finish with the VVG. Regards Tony Reilly Sent from my iPhone > On 10 Mar 2023, at 5:49 am, Akemi Allison via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Just curious, why not do a Movat’s Pentachome? It gives you everything you > are looking for and more. > Best regards, > Akemi Allison-Tacha, BS, Ht, HTL > akemiat3...@gmail.com > > Sent from my iPhone > >> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> Hi, >> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I >> recommend doing the Verhoff elastic stain first, then the Alcian blue, and >> then the Van Gieson. >> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N >> Blue Lake DriveNorman, OK 73069PH >> 405-759-3953http://www.excaliburpathology.com >> >> A sharp knife is nothing without a sharp eye. - Klingon Proverb >> >> On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet >> <histonet@lists.utsouthwestern.edu> wrote: >> >> I am working with a researcher and they saw an article where someone >> performed an Alcian Blue and elastic van Gieson double stain. If anyone >> has done this before can you provide your protocol. I am trying to figure >> out the best way to perform this process to get the best staining results. >> Here are my questions/thoughts >> >> 1. Is it better to run the Alcian blue as usual and then perform the van >> gieson stain? >> 2. Will the differentiating solution in the van gieson stain affect the >> alcian blue staining in any way? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
