The one thing that bothers me about the preloaded programs is the paraffin
temperature is set at 65 degrees C. That's close to 10 degrees higher than the
melting point of our paraffin.
Does anyone know why the temp. is so high? To me, a temp that high will cause
problems with IHC and small biopsies.
Has anyone changed it? Or do folks go along with it just because it's already
there?
I inherited that temp, to change it now would be pain since it is the one we
use the most. If I validated our current Peloris 3, I would have set the wax
temperatures at 60 degrees.
Sincerely,
Paula Sicurello
On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet
<[email protected]> wrote:
Good afternoon Histonet,
We're going to be validating a new tissue processor (Peloris 3) in the coming
months, and I'm curious how people have validated small tissue processing
protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier
to do because we can readily gross duplicate sections. Obviously we can't adopt
this approach for smaller samples because they're entirely submitted. I have a
game plan in mind, but would love some additional input! How'd you do it?
Thanks,
Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
[email protected]<mailto:[email protected]>
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential or
legally privileged information. Any unauthorized review, use, disclosure or
distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of this original
message.
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
