I would also let the saturated solution stand for a few days. Like Oil Red O, it probably needs time to “mature”. I would also use a frozen section of skin as a control.
Regards, Tony Henwood Sydney, Australia From: Bob Richmond via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Wednesday, 29 March 2023 4:51 AM To: Histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Sudanblack B on FFPET > > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. - I've made a saturated Sudan black B-solution in 70% ethanol and tried it with10 min on liver without real success.<< The main thing you need to do is demonstrate that it isn't hemosiderin with an iron stain (Perls prussian blue reaction), and perhaps also that it isn't acid-fast. Lipofuscin can be identified an H & E staining, except for these considerations. Bob Richmond Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet