Hey Monica,


I have not used that exact substitute before, but I do currently use Sub-X
from Leica. It is amazing for running down slides, but I wouldn’t recommend
it for tissue processing as xylene is the best for that.



Attached below Is the xylene substitute and mounting medium I use for
IHC’s.



https://shop.leicabiosystems.com/us/histology-consumables/reagents-solutions/pid-sub-x-clearing-medium

https://www.fishersci.com/shop/products/richard-allan-scientific-mounting-medium-2/22110610



Thanks,



*Lapiana, Anthony, HT(ASCP)*

Laboratory Supervisor, IHC/SPU

Massachusetts General Hospital
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