Hey Monica,
I have not used that exact substitute before, but I do currently use Sub-X from Leica. It is amazing for running down slides, but I wouldn’t recommend it for tissue processing as xylene is the best for that. Attached below Is the xylene substitute and mounting medium I use for IHC’s. https://shop.leicabiosystems.com/us/histology-consumables/reagents-solutions/pid-sub-x-clearing-medium https://www.fishersci.com/shop/products/richard-allan-scientific-mounting-medium-2/22110610 Thanks, *Lapiana, Anthony, HT(ASCP)* Laboratory Supervisor, IHC/SPU Massachusetts General Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet