Hi, I think the answer is as so often: it depends. Detergens solves membranes partly and leads to a higher permeabilization of the tissue. Some antigens may take advantage of that, some may not need it. Higher permeability is good for detection with high molecular complexes.
Gudrun Lang -----Ursprüngliche Nachricht----- Von: Alonso Martínez Canabal via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Mittwoch, 20. September 2023 21:25 An: Histonet Betreff: [Histonet] Detergent in heating antigen retrieval Dear histoneters, I have performed heating antigen retrieval with citrate buffer pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, anyone has any experience or knowledge if it is better with or without the detergent? Thank you! -- Dr. Alonso Martínez Canabal PhD Profesor Asociado "C" Departamento de Biología Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet