Are you allowing them to come up to the temperature of the cryostat before cutting?
-----Original Message----- From: Gerard Spoelstra via Histonet <histonet@lists.utsouthwestern.edu> Sent: Tuesday, September 26, 2023 3:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stored frozen fatty muscle tissue EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hi Histonetters I've been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower the temperature. They are only small biopsies, but are heavily marbled. Any ideas? Gerard Spoelstra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
