1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)
Ken
kdea...@hotmail.com
-----Original Message-----
From: richard cartun via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, January 31, 2024 10:29 AM
To: histonet@lists.utsouthwestern.edu; Foshey, Annette <afos...@mcw.edu>
Subject: Re: [Histonet] Tissue falling off slides
Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633
On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via
Histonet <histonet@lists.utsouthwestern.edu> wrote:
I work with whole mount prostate blocks that I cut and stain for IHC manually.
I use superfrost plus slides that I purchase from the Brain Research
Laboratories. I airdry the slides after sectioning and then they are placed in
a rack and dried in a 60 degree oven overnight. On some of the slides the
tissue still lifts off the slides usually it is where there is tumor. Any
suggestions would be greatly appreciated.
Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afos...@mcw.edu<mailto:afos...@mcw.edu>
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226
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