I agree. Making some of these simple solutions that need to be FRESH is the better way to go. As John said, there are ma ny books with these formulas in them.
I have used Davidson's for different projects and I make mine in the lab fresh each time tissue is harvested. Colleen Forster HT(ASCP)QIHC On Wed, Mar 6, 2024 at 5:26 AM Tony Henwood via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I agree. > > A great book. > > > Regards, > > > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > > Principal Scientist, the Children’s Hospital at Westmead (Retired) > > Adjunct Fellow, School of Medicine, University of Western Sydney. > > ________________________________ > From: John Kiernan via Histonet <histonet@lists.utsouthwestern.edu> > Sent: 06 March 2024 18:32 > To: Histonet <histonet@lists.utsouthwestern.edu>; Naira Margaryan < > nmargarya...@gmail.com>; Davoli, Katherine A <katherine.dav...@pitt.edu> > Subject: Re: [Histonet] Modified Davidson solution > > The Davidson/Hartmann fixative is just another alcoholic formaldehyde > mixture acidified with acetic acid. Like all others of that ilk it was > intended for making up in the lab soon before using. Storage causes slow > deterioration. The ethanol slowly gets esterified by the acetic acid, > making ethyl acetate, which has no fixative properties. This change is > evident from the fruity smell of the ester, which becomes evident after a > week or two. All this has been in the textbooks for more than 50 years. > Why doesn't every lab spend perhaps $200 on a couple of books? For > obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from > the publisher, which is $30 less than Amazon's price), but there are other > histotechnology books that are very good. A book may cost much less than a > big bottle of a deteriorating mixture of questionable value, and you and > your staff could learn a lot by reading. > John Kiernan > = = = > ________________________________ > From: Davoli, Katherine A via Histonet <histonet@lists.utsouthwestern.edu> > Sent: March 5, 2024 4:40 PM > To: Histonet <histonet@lists.utsouthwestern.edu>; Naira Margaryan < > nmargarya...@gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm (they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > ________________________________ > From: Naira Margaryan via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > From: Davoli, Katherine A via Histonet <histonet@lists.utsouthwestern.edu> > Sent: March 5, 2024 4:40 PM > To: Histonet <histonet@lists.utsouthwestern.edu>; Naira Margaryan < > nmargarya...@gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm (they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > ________________________________ > From: Naira Margaryan via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet