Paula, I start my processor at 80%. I am in research and often the samples have been fixed and are put into 70-80% ethanol for holding or transport. Starting with 80% steps right in line with the samples. I rarely see a schedule that starts at 50%. However, I do have a couple labs that ask me for that. I am fortunate to have a second processor I can use for special programs and not hold up my main runs.
Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory On Thu, Mar 28, 2024 at 9:15 AM Paula Sicurello via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Kara, > I do like the VIPs. Besides getting rid of the NBF, the alcohols are > getting rid of the water as well. > I learned EM first, so my processing thoughts are probably biased by that. > Paula > > Yahoo Mail: Search, Organize, Conquer > > On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip<phillip.k...@unthsc.edu> > wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to > use, easy maintenance, and very customizable for programed runs.Every lab I > have been in has always started at 70. It saves the techs extra time mixing > up dilutions because you can always find RTU 70%. It also helps I have > never had any issues with the tissues starting at 70. I get the idea of > starting lower but you are also then going to need to increase your > processing time which can delay turnaround times.Plus correct me if I am > wrong but isn't the whole point of the alcohols after NBF to get the NBF > out of the tissue and ready for xylene and wax? > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w:www.unthsc.edu/corelabs > > From: Paula Sicurello via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Thursday, March 28, 2024 8:04 AM > To: HistoNet <histonet@lists.utsouthwestern.edu> > Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good > Morning, > My question two part: > Best tissue processor ever and why? > What is percentage is your first alcohol step? > I see that lots of places start at 70% but I favor a lower %. One > because it's gentler on the tissue, and two because 10% NBF precipitates > out if you start at 70%. > Thanks for your insight, > Paula > > Yahoo Mail: Search, Organize, Conquer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet