Hartman's (also known as Davidson's) fixative is sometimes used to reveal lymph nodes in resections where they appear opaque - white. It is not surprising that the tissues of the eye would react the same. I assume that the alcohol causes the bleaching of the tissue (or is it the acetic acid?) - more research needed.
Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Davoli, Katherine A via Histonet <histonet@lists.utsouthwestern.edu> Sent: 28 June 2024 04:12 To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] cloudy cornea after Hartman's fix Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative? I'm used to working with mice where, if this happens I didn't notice. Katherine Davoli, MDiv, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
