Hello, Another part of the process is to wick the tissue and place in front of a fan for about 10mins before placing in the 60 degrees oven. Just a suggestion.
Robyn -----Original Message----- From: Catherine Anne Heyward via Histonet <histonet@lists.utsouthwestern.edu> Sent: Thursday, August 8, 2024 5:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re IHC help Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ Hi, If HIER at 90 degrees C is causing tissue lifting, you could try a lower temperature for longer. I was recommended 60 degrees overnight for bone/cartilage and have had far fewer problems with this gentler treatment. Perhaps this would suit the skin samples too. Best wishes, Cath Catherine Heyward Ph.D Senior technician - Oral Research Laboratory Local senior health and safety representative - Faculty for Dentistry Tel: +47 9009 3113 Oral Research Laboratory Institute for Clinical Dentistry Postbox 1109 Blindern 0317 Oslo Geitmyrsveien 69/71 0455 Oslo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!uluFqOasxhMtFqcdQizIWWk3gEV5N-vhhkx-nNFSSY2AuvGV5-cKJhkZfAyZsyMJWRekG8-wVKadQf2eThGlF5vTOY_d9e5cjTWt$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
