Hi Kelly, We measure the pH and document after preparation. We've never had to adjust it; the pH has always fallen within Roche's defined ranges. While the bottle of concentrate itself doesn't mention it, it is printed on the carboy container itself (provided by Roche) and it's in the user manual (page 112 on the version I just looked at). The pH of the diluted wash should be 7.4-7.8. If it falls outside that, contact Roche.
Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357<tel:+13233613357> [email protected]<mailto:[email protected]> ________________________________ From: Pairan, Kelly via Histonet <[email protected]> Sent: Friday, July 11, 2025 8:20:06 AM To: '[email protected]' <[email protected]> Subject: [Histonet] pH of Roche Reaction Buffer (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: [email protected]. Good Morning, How are you pHing the reaction buffer for the Ventana Benchmark Ultra? Roche doesn't have a published pH for diluted reaction buffer. CAP requirement ANP.22500 states that pH must be tested when a new batch is prepared or received. Are you testing the reaction buffer after you dilute the concentrate (ie: preparing a new batch)? Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: [email protected]<mailto:[email protected]> Work: (614) 566-3575 Cell: (614) 312-0104 _______________________________________________ Histonet mailing list [email protected] http://secure-web.cisco.com/1MyA-b1C39589MhhwQL6JkBVVoO2NiPCh_bTw2ge74axqJgmVhSC1NJo0pULgXbc9dTX5XY-URwEdWQOrwcNINcglOY5B4Ec6ouCTPmNSA9Glz1Qp-Pwe18bAGtIRSFF6sUSlDUJPLLfLmBeUDGmnv2guRbUQHdEtdVguiXeSEXuO4E2yHeWpM_oeHHbQqEUgUhAuZUG_8G0FPZoDTF64lX5U5Db3uk9iR7ovOnP3REPFmCQlfsIiX1GT3dOhCL75XGZm7ZucKsCiKTyVepd_vclHreYt2f5a2zi_4DSQ6pFbAdf8L5lGQjJigaWfuGVdG7uKGMKUR9kCOf00dihXEQK9Gqdc0FVnfJ_zUChHrGhA1yhtQb-LIGinhYotFwi755tYwBDWZM_wG_NiKTogMRGWf7AjjkUH8ghqjuTVyih-Z7Ai0yFcvjd2vwGqseDn/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
