Validate all reagent changes. The core principle is that you have introduced an uncharacterized variable to a validated system. Reagent alcohol is typically ethanol denatured with methanol and/or isopropanol. The exact ratio and type of denaturants can differ between vendors. These components are not inert and can affect tissue hydration, dehydration rates, and differentiation of eosin.
Edward Edmonds, HT, QIHC On Wed, Jul 23, 2025, 11:44 AM Pairan, Kelly via Histonet < [email protected]> wrote: > Good Morning, > Our hospital is looking at switching our 100% alcohol and 95% alcohol to > another vendor. I have received mixed answers on whether or not we are > going to need to a validation for this reagent swap when it comes to > processing, staining and IHC. What is the practice at your institution? > > Thanks, > Kelly > > Kelly Pairan, HT(ASCP)CM, HQIPCM > Technical Scientist Anatomic Pathology and Cytology > OhioHealth Laboratory Systems > Suite 210 North Medical Building > 3535 Olentangy River Rd > Columbus, OH 43214 > > Email: [email protected]<mailto:[email protected]> > Work: (614) 566-3575 > Cell: (614) 312-0104 > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
