Insist on a solution made with nuclear fast red powder that has been certified by the Biological Stain Commission. Then you will know the dye really is CI 60760 and also that it works properly as a stain. See https://pubmed.ncbi.nlm.nih.gov/17510813/ [https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/> Certification procedures for nuclear fast red (Kernechtrot), CI 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/> Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has ... pubmed.ncbi.nlm.nih.gov See also https://biologicalstaincommission.org/new-dyes/, and search down the page for the item on nuclear fast red. This includes a warning about products that are sold as "certified" stains but have not been tested by the BSC.
Hoping this helps. John Kiernan. = = = ________________________________ From: Histology via Histonet <[email protected]> Sent: Tuesday, August 19, 2025 12:31 PM To: Histonet <[email protected]> Subject: [Histonet] Nuclear fast red counterstain Hi all, Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it? We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide. We have tried filtering the solution but we are still seeing this problem. Any help would be much appreciated. Thanks as always Histonet!! Mehndi Helgren Lab Manager 757-664-7901 Dominion Pathology Labs. 733 Boush St. Suite 200 Norfolk, VA 23510 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
