Be sure stainer is not expired John IMEB Inc Fresh stain less then 1 year old is best option we find
> On Aug 20, 2025, at 10:09 AM, [email protected] wrote: > > Send Histonet mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Nuclear fast red counterstain (Histology) > 2. Re: Nuclear fast red counterstain (John Kiernan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 19 Aug 2025 17:31:00 +0000 > From: Histology <[email protected]> > To: Histonet <[email protected]> > Subject: [Histonet] Nuclear fast red counterstain > Message-ID: <[email protected]> > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > Has anyone experienced commercially pre-made Nuclear Fast Red having reddish > crystals in it? We use this as a counterstain for Prussian Iron and Melanin > stains and we are seeing little crystals on the slide. We have tried > filtering the solution but we are still seeing this problem. Any help would > be much appreciated. > > Thanks as always Histonet!! > > Mehndi Helgren > Lab Manager > 757-664-7901 > Dominion Pathology Labs. > 733 Boush St. Suite 200 > Norfolk, VA 23510 > > > > ------------------------------ > > Message: 2 > Date: Tue, 19 Aug 2025 19:32:29 +0000 > From: John Kiernan <[email protected]> > To: Histology <[email protected]>, Amrit Samra via Histonet > <[email protected]> > Subject: Re: [Histonet] Nuclear fast red counterstain > Message-ID: > > <yt3pr01mb1082091292c9e6c777c08005ba5...@yt3pr01mb10820.canprd01.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Insist on a solution made with nuclear fast red powder that has been > certified by the Biological Stain Commission. Then you will know the dye > really is CI 60760 and also that it works properly as a stain. See > https://pubmed.ncbi.nlm.nih.gov/17510813/ > [https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/> > Certification procedures for nuclear fast red (Kernechtrot), CI > 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/> > Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly > used in conjunction with an excess of aluminum ions as a red nuclear > counterstain following histochemical procedures that yield blue products. The > dye has also been used as a histochemical and colorimetric reagent for > calcium. Unsatisfactory samples of nuclear fast red are encountered > occasionally, and confusion has ... > pubmed.ncbi.nlm.nih.gov > See also https://biologicalstaincommission.org/new-dyes/, and search down > the page for the item on nuclear fast red. This includes a warning about > products that are sold as "certified" stains but have not been tested by the > BSC. > > Hoping this helps. John Kiernan. > = = = > ________________________________ > From: Histology via Histonet <[email protected]> > Sent: Tuesday, August 19, 2025 12:31 PM > To: Histonet <[email protected]> > Subject: [Histonet] Nuclear fast red counterstain > > Hi all, > > Has anyone experienced commercially pre-made Nuclear Fast Red having reddish > crystals in it? We use this as a counterstain for Prussian Iron and Melanin > stains and we are seeing little crystals on the slide. We have tried > filtering the solution but we are still seeing this problem. Any help would > be much appreciated. > > Thanks as always Histonet!! > > Mehndi Helgren > Lab Manager > 757-664-7901 > Dominion Pathology Labs. > 733 Boush St. Suite 200 > Norfolk, VA 23510 > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 261, Issue 5 > **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
