> > Good evening, > > > > At the end of the day, your SOP should spell out what to do in this > situation. Let that document, signed off by your technical manager or > director, guide the call. > > Here’s my take (strictly my opinion, not your lab’s): > > If you’re using a pure solvent like ethanol or isopropanol, or Xylene. > the CAS number doesn’t change by vendor. > > 1. If the reagent is part of the stain’s analytical method: (H&E > stain), then a small comparison study is usually all that’s needed since > the reagents being switched are exactly the same in their properties & > concentrations. The only change is the distributor/vendor the reagent is > being acquired from. > > 2. If the reagent is *Not* part of the stain’s analytical method: > If the alcohol and xylene aren’t method steps for performing the test > (such as being the dehydration and clearing steps only) then I wouldn’t > consider that revalidation-worthy. > > > > I hope this helps! > > > > Best regards, > Eddie Martin > Technical Pathology Specialist > The National Institutes of Health > 10 Center Drive > Building 10, RM 2C301 > Bethesda, MD 20816 > (301) 594-2054 > [email protected] >
> On Wed, Jul 23, 2025, 11:44 AM Pairan, Kelly via Histonet < > [email protected]> wrote: > > > Good Morning, > > Our hospital is looking at switching our 100% alcohol and 95% alcohol to > > another vendor. I have received mixed answers on whether or not we are > > going to need to a validation for this reagent swap when it comes to > > processing, staining and IHC. What is the practice at your institution? > > > > Thanks, > > Kelly > > > > Kelly Pairan, HT(ASCP)CM, HQIPCM > > Technical Scientist Anatomic Pathology and Cytology > > OhioHealth Laboratory Systems > > Suite 210 North Medical Building > > 3535 Olentangy River Rd > > Columbus, OH 43214 > > > > Email: [email protected]<mailto:[email protected]> > > Work: (614) 566-3575 > > Cell: (614) 312-0104 > > > > _______________________________________________ > > Histonet mailing list > > [email protected] > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
