Hi Michael,
the problem with image size is probably related to the memory you have
allocated to ImageJ.
ImageJ has a limit for the number of pixels in an image of 2^31 - 1,
roughly 2.14e9. Your image size is 58157 x 33163 = 1.93e9, so in
principle it should be possible to open it.
With 4 channels, 2 bytes each, the image needs 14 GB of memory, plus 7
GB (4 bytes/pixel) to display it. If you want to do any operations on
the image you will need more memory. I think that 32 GB RAM with 30 GB
or so allocated to ImageJ (if no other program is open) would be the
minimum for very basic operations; many operations will need more.
Michael
________________________________________________________________
On 11.03.24 18:10, Cammer, Michael wrote:
We are wondering whether anybody has solved the following problem?
We have time lapse images of a few fields of stitched images and also still
images of much larger stitched images (whole mouse brain slices) generated with
Nikon Elements which we cannot open in Fiji. We have tried both BioFormats
6.5.0 and 7.2.0.
The error we get is "Unsupported format or file not found."
When ND2 did not work, we tried exporting to tif files. We have tried both
separating the channels to generate smaller single channel images and saving at
OME TIFF. Both yield the same error message.
We did try cropping the largest of our images, 58157 x 33163 pixels, exactly in
half, but this will not open too (29078 x 33163 pixels, 4 16 bit channels). We
tried cropping out a small area, 2000 x 2000 pixels, and this does open ok. We
need two of the 4 channels in 16 bits, so we did not try converting to 8 bits.
This led us to the question, what is the largest image that BioFormats in
ImageJ will open from a Nikon Elements generated dataset? Have we hit a
ceiling?
Any help would be appreciated.
Best regards-
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, New York, NY 10016
[email protected]<mailto:[email protected]>
http://nyulmc.org/micros http://microscopynotes.com/
Cell voice (no text) 1-914-309-3270
Office 1-646-501-0567
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