I was trying to convert an image back to RGB after using run("Make Composite")
and ran into a problem. Instead of converting the image as shown to RGB, the
channels were reassigned RGB in the original order. In this example run on my
computer, the red and green channels should be swapped, but the resulting RGB
image has them not swapped. Is this expected behavior or a bug?
1.54k11
Thank you!
// Creates a test RGB Image. Instead, use any existing RGB image.
newImage("Test HyperStack", "8-bit color-mode", 512, 256, 3, 1, 1);
setSlice(1);
makeRectangle(23, 27, 139, 182);
run("Set...", "value=255 slice");
setSlice(2);
makeRectangle(126, 44, 139, 182);
run("Set...", "value=255 slice");
setSlice(3);
makeRectangle(234, 70, 139, 182);
run("Set...", "value=255 slice");
Property.set("CompositeProjection", "Sum");
Stack.setDisplayMode("composite");
run("Select None");
run("RGB Color");
//swap channels and convert to RGB
run("Make Composite");
setSlice(1);
run("Green");
setSlice(2);
run("Red");
waitForUser("Red and green should be swapped");
run("RGB Color");
// The resulting image I get is the original RGB image, not the one with
channels swapped.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
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