Hi Kenneth, Not sure what you mean. If I open Confocal Series I see pixel_width=0.0544550 pixel_height=0.0544550. If I change the image size from 400 pixels to 200 pixels I get pixel_width=0.1089100 pixel_height=0.1089100, so the calibration has been adjusted.
Best wishes Kees Advanced Imaging Facility (RRID: SCR_020967) University of Leicester www.le.ac.uk/advanced-imaging-facility<http://www.le.ac.uk/advanced-imaging-facility> ________________________________ From: Kenneth Sloan <[email protected]> Sent: 15 August 2024 02:16 To: [email protected] <[email protected]> Subject: Calibration feature request ***CAUTION:*** This email was sent from an EXTERNAL source. Think before clicking links or opening attachments. I have been making more and more use of the Calibration feature to establish custom coordinate systems for images. I note that when an image is re-sized (and presumably cropped, although I haven't tested this yet), the Calibration remains the same. Is it possible for operations such as "resize" to update the Calibration, if one is attached to an image? It seems obvious to me (perhaps it's not obvious?) that xOrigin, yOrigin, pixelHeight, pixelWidth can all be modified in a straightforward way. -- Kenneth Sloan [email protected] Vision is the art of seeing what is invisible to others. -- ImageJ mailing list: https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fimagej.nih.gov%2Fij%2Flist.html&data=05%7C02%7Ckrs5%40leicester.ac.uk%7C26981c16b67f4e2817db08dcbcc8242b%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C0%7C638592815007963204%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=RNFdp%2BxtNCBEHNcnN2UXEg8Oc4Xp3qe43ypXbxSIkzU%3D&reserved=0<http://imagej.nih.gov/ij/list.html> -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
