Dear Dipanjana, I guess what you need is an ad hoc macro. The best would be that you make 1-2 example picture available and better explain what you really need. My best regards, Philippe
Philippe CARL Laboratoire de Bioimagerie et Pathologies UMR 7021 CNRS - Université de Strasbourg Faculté de Pharmacie 74 route du Rhin 67401 ILLKIRCH Tel : +33(0)3 68 85 41 41 ----- Mail original ----- De: "Dipanjana Ghosh" <[email protected]> À: "imagej" <[email protected]> Envoyé: Samedi 17 Août 2024 08:03:57 Objet: Object based intensity calculation using ImageJ Dear all, We want to calculate the intensities of a channel where objects (puncta) are detected. The intensities from the puntas are to be calculated applying some threshold for size and intensities. Can someone kindly explain which tool of imageJ can be used for this and how? Thanking you in advance Regards, Dipanjana -- Dr. Dipanjana Ghosh DST INSPIRE Faculty School of Biomolecular Engineering & Biotechnology RGPV, Bhopal ORCID: https://orcid.org/0000-0003-0887-6798 Prev: DST WOS-A, IISERB Post doctoral Research Fellow Department of Biological Sciences National University of Singapore Singapore -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
