Dear Herbie, Thank you for your prompt response and helpful suggestions. As I am relatively new to ImageJ, I have a follow-up question regarding a related scenario.
What if one has a selected *.mrc stack of cropped cryo-EM particles (e.g., liposomes) that have been corrected using a Wiener filter. Would it be possible to use ImageJ to perform batch analysis and measure different parameters on this stack? Is it simplifying the problem? Thank you for your time. Kind regards, Dmitry ________________________________ From: Herbie <[email protected]> Sent: 28 November 2024 18:36 To: [email protected] <[email protected]> Subject: Re: Inquiry on Analyzing Liposomes in Cryo-EM Images Greetings Dmitry, in order to be able to substantially provide some help, we need to see typical images in a non-lossy file-format, preferably TIF . "advice on how to address defocus in these images" There is only one generic method which is "(adaptive) Wiener-corrected inverse filtering". AI-approaches *at best* will produce comparable results. Building good inverse filters is far from trivial because they strongly depend on the individual image statistics (S/N-ratio): <https://www.gluender.de/Writings/WritingsTexts/WritingsDownloads/1980_NoiseInDeblurring_scan.zip> Regards Herbie ::::::::::::::::::::::::::::::::::::::::::::: Am 28.11.24 um 19:16 schrieb Dmitry Semchonok: > Dear colleagues, > I hope this message finds you well. > > I am seeking guidance on the proper methods for analyzing liposomes in > cryo-electron microscopy (cryo-EM) images. Specifically, I have some raw > cryo-EM images (*.mrc, *.eer) containing liposomes, and I would like to > determine their diameter, area, and circularity etc. > Is there a batch script available that can facilitate the measurement of > these parameters and assist in preparing the necessary statistics? > Additionally, I would appreciate any advice on how to address defocus in > these images. > > Thank you for your assistance. > Kind regards, > Dmitry -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
