Dear colleague,

please understand that

  "The result you attached is close to what I aim for."

is not really helpful. If my result is only *close* to what you like to see, then please tell us what is missing?

"I aim to align the objects in each slice [...] to the same position as
they appear in the first slice."

This task is ill-posed because the noisy shape of the objects differs considerably. Without additional information (reference lines or points) the only way to align the objects is to make assumptions about the meaning of >>aligning differently shaped noisy objects<<. My approach is based on a common assumption (see my reply to the post of Jeff Stokes).

It isn't clear if you consider alignments in x and y, or only in y. My result uses y-alignment only but it is easy to do the x-alignment as well.

Please be more specific!

Regards

Herbie

:::::::::::::::::::::::::::::::::::::::::::::
Am 06.08.25 um 23:01 schrieb AR.M. AlHinnawi:
Thank you.

The result you attached is close to what I aim for.

Could you please explain how or what ImageJ tools you used? Is it possible to put it in a loop in a macro?

To explain the problem further, I aim to align the objects in each slice of 400 slices (like the result you sent) to the same position as they appear in the first slice. I hope this helps explaining the problem that I am trying to solve..

Thank you again.

/*Abdel-Razzak Al-Hinnawi, (Full Prof.) (B.Sc., M.Sc., Ph.D. Medical Imaging  Sciences & Digital Image Processing & Biomedical Engineering)*/
/*Faculty of Allied Medical Sciences*/
/*ISRA University*/
/*11622, Amman, *//*JORDAN*/
/*phone: +962 780515199*/
/*e-mail: *[email protected] <mailto:[email protected]>[email protected] <mailto:[email protected]>_/


On Wed, 6 Aug 2025 at 23:48, Gluender <[email protected] <mailto:[email protected]>> wrote:

    Greetings,

    attached please find the best I can get for your sample images.

    BTW, your description of what you want isn't really helpful.

    Regards

    Herbie

    :::::::::::::::::::::::::::::::::::::::::::::
    Am 06.08.25 um 21:23 schrieb AR.M. AlHinnawi:
     > Dear ImageJ
     >
     > I have a stack of 400 images. They all show the same objects but at
     > different settings, resulting in irregular displacements.
     >
     > The interested objects can be confined in a rectangular area in
    the first
     > slice (a rectangular area that can be drawn manually).
     >
     > I aim to make similar objects in all remaining slices to fit in the
     > rectangle. For example, I attached the first and last slice.
     >
     > It doesn't seem easy.
     >
     > Please, can you advise?
     >
     > Thank you
     >
     > *Abdel-Razzak Al-Hinnawi, (Full Prof.)  (B.Sc., M.Sc., Ph.D. Medical
     > Imaging  Sciences & Digital Image Processing & Biomedical
    Engineering)*
     > *Faculty of Allied Medical Sciences*
     > *ISRA University*
     > *11622, Amman, **JORDAN*
     > *phone: +962 780515199*
     > *e-mail: [email protected] <mailto:[email protected]>
    <[email protected] <mailto:[email protected]>>
     > [email protected]
    <mailto:[email protected]>
    <[email protected] <mailto:[email protected]>>*
     >
     > --
     > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
    <http://imagej.nih.gov/ij/list.html>
     >



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