Greetings Lance,
did you try
selectImage("Mock");
roiManager("Select", 0);
Roi.setStrokeWidth(2);
roiManager("Multi Plot");
instead?
Best
Herbie
:::::::::::::::::::::::::::::::::::::::::::::
Am 07.08.25 um 20:13 schrieb Davidson, Lance:
Hi All,
Recent efforts to use plotprofile with different linewidths have given some
surpising results where the length changes with the linewidth.
We are using free line ROIs automatically generated by segmentation algorithms.
I've simplified the macro below to do the same thing with a simple two-segment
polyline.
If the line is 1 pixel wide the reported distance is 685 pixels, if the line is
2 pixels wide the distance is 705...
Not only is the plotprofile outcome odd, but the polyline gets "splined".
Any help or workaround would be appreciated.
Best,
Lance
Lance Davidson, MSc, PhD
William Kepler Whiteford Professor of Bioengineering
University of Pittsburgh
//---------------------- offending macro -----------------
newImage("Mock", "16-bit black", 850, 850, 1);
makeLine(156,486,392,255,683,459);
roiManager("Add");
selectImage("Mock");
roiManager("Select", 0);
Roi.setStrokeWidth(1);
run("Plot Profile");
selectImage("Mock");
roiManager("Select", 0);
Roi.setStrokeWidth(2);
run("Plot Profile");
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