Very valuable and useful info. Thanks for sharing.

On May 11, 2:10 pm, Satish Phadke <drsmpha...@gmail.com> wrote:
> The link may not work so I have copied the contents of an article related to
> one interesting plant we had discussed earlier. I think Pankaj Oudhia ji has
> more knowledge on 
> this.http://epaper.timesofindia.com/Default/Client.asp?Daily=TOIPU&showST=...
>
> http://epaper.timesofindia.com/Default/Scripting/ArticleWin.asp?From=...
>
> Dr Phadke Satish
>
> Green group conducts DNA barcoding of Ramkand Kalyani Sardesai TNN
>
> Pune: Sahyadri Genes, a Kolhapur-based environmentalcum-research group, and
> the department of botany, Shivaji University, have carried out DNA barcoding
> of the Ramkand plant, which, according to popular legend, was consumed by
> Lord Rama while in exile.
>     The study, which establishes that the plant is a monocot and not a
> tuber, has been published in the recent issue of ‘Current Science’.
>     Speaking to TOI, Mansingraj Nimbalkar, president, Sahyadri Genes, said,
> “For several years, the exact identity of Ramkand has remained a mystery for
> both plant researchers and students. Though the tuber is being sold for
> several years, especially at places of pilgrimage, its source is one of the
> best kept secrets by vendors. The name and information provided by vendors
> give an impression that the tuber was eaten by Lord Rama during his days of
> exile. Previous efforts to identify the plant have proved unsuccessful. The
> only material available for study are the thin slices sold by vendors.”
>     During the study, the team initially found it difficult to establish its
> identity as a monocot. “Though the anatomical study showed a cell structure
> typical to the monocot, it only confused us further. This is because,
> monocots have adventitious roots and not a tap root.”
>     The DNA was extracted from the slices obtained from a vendor at Jyotiba
> hill temple at Wadi Ratnagiri in Kolhapur district. The slices were
> approximately 4.5 inches in size and two to three mm in thickness.
>     The sequence was used to find similarities with the other submitted
> sequences. The search showed that the sequence was 89 per cent similar to
> the Agave sisalana — a monocot.
>     “To confirm this further, we checked more plants of the Agave genus. The
> leaves enclosing the rosette and juvenile inflorescence (soft middle portion
> of the plant) were removed, which exposed the core of the rosette. The core
> was soft and similar in dimension to that of the Ramkand plant,” said G B
> Dikshit, professor, department of botany, Shivaji University.
>     Taxonomist S R Yadav explained the significance of the DNA barcoding.
> “It is a major step forward as the plant has proved to be difficult to
> place. Over the last 25 years, I have approached leading botanists in the
> country to help me identify the Ramkand, but they could not,” he said.
>     Moreover, the study dispels the myth about the plant being a tuber. “The
> origin of the plant is being carefully concealed by vendors, but Ramkand,
> which is sold to devotees in the name of Lord Rama, is actually not
> recommended for human consumption. In fact, it can prove to be poisonous,”
> he said.
>     “One variety of the Agave genus is used to make a syrup for diabetic
> patients, but, by and large, the species are not meant to be eaten. Since it
> is sold in thin slices and people consume it in small quantities, it may not
> have had any noticeable after-effects. But it is important that people know
> the facts,” Yadav said.
>
> The Ramkand plant

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