Hey scientist, Are you using anything related to GPIB/Serial? You posted the question talking about the NI-488 error, but really I think the error you are receiving is more pertinent to the LabVIEW: File not found error. Errors in LabVIEW generally have two possible causes - one is what the error means if LabVIEW caused it, the other is what it means if it was related to the NI-488.2 driver.
So you get a file not found error anytime you try and open any (????) file in LabVIEW 6.1? Does that mean when you try and open a VI in 6.1, or when you use a File I/O VI inside of a 6.1 VI? Can you open that same file up using LabVIEW 7.0 and 7.1? What is the type of file that you are trying to open, and where is it located? JaredA