I can't see using a fret calculator for real world tuning, tune your open strings in the temperament you wish and then set your frets using the tuning box. If you wish to tweak the open strings, tune the top string, then set each fret with the box. Then tune the double octave to the top string, set those frets to the box. You will notice that the frets do not make exactly straight lines, which is normal--and shows that the fret calculator won't work. Then adjust the open strings anyway you like. You have to have the double octaves in tune or you will really hear it. This is the only system that will give you the actual, as opposed to theoretical values, as it will compensate for drift--unless you tune by ear. You will find, of course, that you will either have to choose between sharps and flats, especially for the first fret, or use tastini.
The fret caluclator will get you close, but still a few millimeters from cigar. Have fun! dt To get on or off this list see list information at http://www.cs.dartmouth.edu/~wbc/lute-admin/index.html