Dear Colleagues,

My coauthors and I are excited to share our new publication in Journal of
Comparative Neurology:


Avelino-de-Souza, K., N. Patzke, K. Æ. Karlsson, P. R. Manger, and S.
Herculano-Houzel. 2025. “ Cellular Composition of the Brain of a Northern
Minke Whale.” *Journal of Comparative Neurology* 533, no. 9: e70089.
https://doi.org/10.1002/cne.70089


*Abstract*
The largest mammalian brains belong to cetacean species among the
cetartiodactyls. Stereological analyses have estimated cetacean numbers of
cerebral cortical neurons to be more than the average 16 billion of humans,
yet isotropic fractionator estimates in artiodactyls predict that even the
largest cetacean brains should have no more than a few billion cortical
neurons. Here, we used the isotropic fractionator to investigate these
contrasting estimates of neuronal numbers by determining the numbers of
neurons and non-neuronal cells forming the brain of a northern minke whale,
previously estimated using stereology as containing 12.8 billion cortical
neurons (Eriksen and Pakkenberg 2007), and comparing it to our dataset of
several dozen mammalian species analyzed with the same method. We report
that, with 3.2 billion neurons, the minke whale cerebral cortex conforms to
the quantitative scaling rules that apply to other mammals, especially the
closely related artiodactyls. The same brain contained a total of 57.4
billion neurons, of which 54.2 billion were cerebellar neurons, matching
the expected numbers of a hypothetical artiodactyl brain of similar
cerebellar mass. In addition, we found that the northern minke whale brain,
with a mass of 2683.9 g, contained 173.4 billion non-neuronal cells,
following the universal scaling rules that apply to the brain in all
therian mammals examined to date. Thus, how non-neuronal cells are added to
the mammalian brain is conserved across therian mammals and is not affected
by the transition to an obligatory aquatic life history. Strikingly, we
find that the minke whale is an outlier amongst mammals in having almost 18
cerebellar neurons for every neuron in the cerebral cortex, compared to the
average ratio of 4, which might be related to infrasonic communication. In
addition, with only approximately 88 million neurons, the remainder of the
brain (brainstem/diencephalon/subcortical telencephalon) of the northern
minke whale exhibited the lowest relative neuronal density of these regions
reported in mammalian brains, which might be related to the absence of
limbs compared to all other mammalian species. Our results indicate that
the number of neurons in cetacean brains has been grossly overestimated by
stereological accounts, and place whale brains on par with highly
cognitively capable macaws, macaques, baboons, and elephants, but below
great apes and humans, in terms of numbers of cortical neurons.



Best,

-- 
*| Kamilla A. de Souza*

Director of the Brazilian Neurobiodiversity Network
Ph.D. in Morphological Sciences
Federal University of Rio de Janeiro, Brazil.
[email protected]
+5521 999261442
*https://lattes.cnpq.br/0983547349106771*
<https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=EBC24F10BBACA52C80481320E9E56792#>
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