Hi Allyson,

looking at your query, seems you are using UNIPROT PROTOTYPE which is quite out of date and so is GO data in UNIPROT PROTOTYPE. The new and comprehensive version of UNIPROT is about to be released (Jie cc'ed here can point you to the test site too). Also, I guess the attributes you are interested in can be retrieved from Ensembl too, please try that.

Thanks
Syed


Allyson Lister wrote:
Hi Syed,

I guess I'm still not following. I select "All" rows, and I do get a longer list, but still *every cell* in the GO ID column says "GO:0007049". Now, this ID is for "cell cycle" (see http://amigo.geneontology.org/cgi-bin/amigo/term-details.cgi?term=GO:0007049&session_id=6322amigo1232721760 <http://amigo.geneontology.org/cgi-bin/amigo/term-details.cgi?term=GO:0007049&session_id=6322amigo1232721760>). This implies to me that there is a problem in the display of the martview, and that it is displaying this one GO ID for all go terms that fit the query. Or, there could be a problem in the results of the query, which would be more of a problem.

The display as shown below when the GO Description is included is wrong, I'm sorry to say. It should have different go ids for each row.

Does this explain what I'm getting at a little better?

Thanks for being so quick to reply!

:)

2009/1/23 Syed Haider <[email protected] <mailto:[email protected]>>

    Hi Allyson,

    I guess its just because that you are looking at the snapshot of
    data. If you retrieve the complete results set, that will be the
    same in terms of first three columns. Please try that and let me
    know if that explains...

    Cheers
    Syed


    Allyson Lister wrote:

        Hi Syed,

        I'm not sure that is the reason, because even though the GO
        IDs *seem* identical, the GO Descriptions, as seen in the
        image, are all different. Indeed, when I add the unique
        results only flag, I get the exact same result.

        Seeing as the image didn't seem to have been passed, here is a
        bad cut-and-paste of the table (irrespective of the unique
        results flag):

        P32797      GO:0007049      CDC13      cell cycle
        P32797     GO:0007049     CDC13     physiological process
        P32797     GO:0007049     CDC13     cellular process
        P32797     GO:0007049     CDC13     cellular physiological process
        P32797     GO:0007049     CDC13     intracellular
        P32797     GO:0007049     CDC13     cell
        P32797     GO:0007049     CDC13     chromosome
        P32797     GO:0007049     CDC13     intracellular
        non-membrane-bound organelle
        P32797     GO:0007049     CDC13     non-membrane-bound organelle
        P32797     GO:0007049     CDC13     intracellular organelle*

        *Weirdly, when I remove the "GO Description" attribute, as I
        said in my first email, I get a result where the GO IDs seem
        to be properly displayed.

        P32797      GO:0007049      CDC13
        P32797     GO:0007582     CDC13
        P32797     GO:0009987     CDC13
        P32797     GO:0050875     CDC13
        P32797     GO:0005622     CDC13
        P32797     GO:0005623     CDC13
        P32797     GO:0005694     CDC13
        P32797     GO:0043232     CDC13
        P32797     GO:0043228     CDC13
        P32797     GO:0043229     CDC13

        So, what do you think is going on here?

        Thanks! :)

        2009/1/23 Syed Haider <[email protected] <mailto:[email protected]>
        <mailto:[email protected] <mailto:[email protected]>>>


           Hi Allyson,
           I am not sure why it dint reach mart-dev earlier on. The
        history
           is available here:

http://listserver.ebi.ac.uk/mailing-lists-archives/mart-dev/threads.html

           May be it never made it to the list because you were sending an
           attachment, and its not possible to send attachments to the
        list :)

           Anyways, I guess the reason you see many GO IDs when you select
           Description attribute is because of redundant rows against
        several
           Gene IDs and associated GO IDs. The best is to try
        UniqueRows flag
           and see if that removes the irrrelevant rows.

           Cheers
           Syed




               ---------- Forwarded message ----------
               From: *Allyson Lister* <[email protected]
        <mailto:[email protected]>
               <mailto:[email protected]
        <mailto:[email protected]>>
               <mailto:[email protected]
        <mailto:[email protected]>
               <mailto:[email protected]
        <mailto:[email protected]>>>>
               Date: 2009/1/16
               Subject: interpreting GO IDs
               To: "[email protected] <mailto:[email protected]>
        <mailto:[email protected] <mailto:[email protected]>>
               <mailto:[email protected] <mailto:[email protected]>
        <mailto:[email protected] <mailto:[email protected]>>>"
               <[email protected] <mailto:[email protected]>
        <mailto:[email protected] <mailto:[email protected]>>
               <mailto:[email protected] <mailto:[email protected]>
        <mailto:[email protected] <mailto:[email protected]>>>>



               Not sure if this made it to the mailing list the first
        time,
               so sending again. Apologies if you get it twice!




               Hi all,

               I've just run this query on the biomart.org
        <http://biomart.org>
               <http://biomart.org> <http://biomart.org> martview
        application:



               <?xml version="1.0" encoding="UTF-8"?>
               <!DOCTYPE Query>

               <Query  virtualSchemaName = "default" formatter = "TSV"
        header
               = "0" uniqueRows = "0" count = "" datasetConfigVersion
        = "0.5" >
                                                    <Dataset name =
        "uniprot" interface = "default" >



                              <Filter name = "gene_name" value = "cdc13"/>
                              <Attribute name = "sptr_ac" />
                              <Attribute name = "go_id" />
                              <Attribute name = "gene_name" />



                              <Attribute name = "go_name" />
                      </Dataset>
               </Query>

               And I get the attached result (screenshot). I may be
        extremely
               dense here, but why are all of the GO IDs the same for the
               first few GO descriptions? When I just choose GO ID
        (and not
               also GO description in the Attributes section, I get a
        variety
               of GO IDs.

               Anyone know what I'm doing wrong in querying or
        interpreting?

               Many thanks!

               --
               Allyson Lister
               http://lurena.vox.com

               CISBAN, http://www.cisban.ac.uk
               Newcastle University



               --
               Allyson Lister
               http://lurena.vox.com

               CISBAN, http://www.cisban.ac.uk
               Newcastle University



               --
               Allyson Lister
               http://lurena.vox.com

               CISBAN, http://www.cisban.ac.uk
               Newcastle University

------------------------------------------------------------------------




--
        Allyson Lister
        http://lurena.vox.com

        CISBAN, http://www.cisban.ac.uk
        Newcastle University




--

Allyson Lister
http://lurena.vox.com

CISBAN, http://www.cisban.ac.uk
Newcastle University

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